ZEISS & Wiley present an exclusive interactive White Paper collection that discusses the challenges of automated microscopy
Often in life sciences research, the data you are after will only be revealed through multiple runs of experiments or complex assays. Automation and parallelization of your imaging can be the only way to get there. Observing live samples over a number of days or imaging lots of multiwell plates really puts your microscope through its paces.
To get reproducible, unbiased data, you must control environmental conditions such as light, temperature and CO2. Especially for demanding long-term timelapse fluorescence imaging, you need gentle illumination and a stable platform.
This collection of White Papers aims to give you insights into the challenges and recent developments in the ever-expanding world of automated microscopy solutions. Automation can simplify your laboratory setup and make your work more efficient and comfortable.
Abstracts & Authors:
The Challenge: The Long Way from Motorization to True Automation in Imaging
by Horst Wolff, ZEISS Microscopy, Munich
Rapid developments at all levels of microscopy, such as contrast, illumination, resolution, signal detection and data processing have occurred over the last decades and there is reason to expect that these advances will continue. However, severe limitations in accuracy, reproducibility and throughput are caused by the involvement of humans in all steps of the imaging workflow. It also poses a significant burden and workload for the researcher. To improve this situation is the biggest challenge in automation.
High Throughput Live Cell Imaging: New Opportunities and Challenges
by Rainer Pepperkok et al., EMBL, Heidelberg
High throughput microscopy of fixed samples has been extensively used in the past to characterize gene function at the genome scale with excellent single cell resolution. High throughput live cell imaging, which can provide essential information about system dynamics in single cells bears additional challenges and has thus been used in only a few cases for genome scale experiments. Here we describe latest developments of the technology focussing on high throughput live cell imaging and feedback microscopy with ZEISS Celldiscoverer 7 and LSM 780.
The study of arthropod embryogenesis can provide insight into the evolution of development mechanisms. Many aspects of development are most effectively studied by live-imaging the development of many embryos. Historically, this has been difficult, but new tools are making the collection of such datasets easier than ever.
Big Microscopy Dataset and File Management: Examples of Three Workflows Implemented at the FMI in Basel
by Laurent Gelman, FMI, Basel
New microscopy modalities, e.g. live-cell imaging, slidescanning, high-content screening and 3D-electron microscopy, associated to biological projects aiming at more quantitative data, generate datasets which are one to several orders bigger than before. To cope with this exponential growth, new workflows and important investments in IT solutions are needed. Unfortunately, there is no single workflow, nor a single computer configuration that can do it all. I present here three different workflows to exemplify the issues and some solutions that have been found in our institute.
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