ZEISS EMBL3D Talk: Making Light Work of 3D Bioimaging, by Peter O’Toole

May 23, 2016
3D Bioimaging arguably came to life with the confocal microscope era and we continue to develop microscopy to help address more complex questions. Advances include these 05 examples: The standard confocal microscope that readily enables optical sectioning of fluorescently stained samples. The advent of GFP which permits 3D imaging of more complex live samples and studies of individual proteins and multicellular dynamics. Multiphoton microscopy that takes us deeper into in vivo samples. Increases in detector sensitivity which has helped lower toxicity for live cell experiments and increases the available speed to vitesse temporal issues. Light Sheet microscopy that gives us the ability to image whole organisms over time with minimal perturbation. Together, we now have a huge arsenal of techniques in which to help visualise and inform our fundamental understanding of biology and how cells work. http://www.selectscience.net/embl-3d-microscopy | http://www.zeiss.com/microscopy
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