ZEISS Celldiscoverer 7: Human Embryonic Kidney cells expressing eGFP, 5 day time-lapse microscopy

November 2, 2016
GFP HEK (Human Embryonic Kidney) cells, transiently expressing eGFP, imaged through a 1 mm plastic bottom dish. Images taken every 5 minutes for a total of 5 days. Imaging started shortly after induction of the expression via Tetracyclin treatment. Overlay of phase gradient contrast and green (eGFP) fluorescence. After one day: cells are subconfluent and start to express eGFP. Due to the transient transfection and the Tetracyclin treatment some round and dead cells are visible. After two days, cells have recovered from the transfection and start to grow again. At the end of the time series, cells are confluent and bright green due to eGFP expression. http://www.zeiss.com/celldiscoverer Sample courtesy of Sanofi-Aventis Deutschland GmbH, R&D IDD / in vitro Biology, Frankfurt, Germany.
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