ZEISS Celldiscoverer 7: Cricket embryo, 96 hours time-lapse fluorescence microscopy

November 2, 2016
Five days long-term imaging of cricket embryogenesis. The development of an eGFP-expressing cricket embryo mounted in low melt agarose was imaged every 5 minutes for a total length of 5 days. During the first day the retraction of the yolk and dorsal closure can be seen followed by further 500 μm growing of the embryo. EDF-images created from Z-stacks; acquired with 2.5 x magnification using short exposure times of 35 ms. Z-stacks acquired within 2.3 seconds and are 350 μm thick. http://www.zeiss.com/celldiscoverer Sample courtesy of S. Donoughe, BioLabs Building 2087, Harvard University, Cambridge, USA
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