The Journal of Visualized Experiments (JoVE) is a PubMed-indexed video journal with the mission to increase the productivity of scientific research. Our article features a detailed video protocol for live confocal imaging of developing Arabidopsis flowers with ZEISS stereo and laser scanning microscopy systems. Enjoy the video protocol and let yourself be inspired!
Department of Biology and Biological Engineering, California Institute of Technology
Live confocal imaging provides plant biologists with a powerful tool to study development, and has been extensively used to study root growth and the formation of lateral organs on the flanks of the shoot apical meristem. However, it has not been widely applied to the study of flower development, in part due to challenges that are specific to imaging flowers, such as the sepals that grow over the flower meristem, and filter out the fluorescence from underlying tissues. Nathanaël Prunet presents a detailed protocol to perform live confocal imaging on live, developing Arabidopsis flower buds, using either an upright or an inverted microscope.
For more images of fluorescent flowers imaged with confocal microscopy, visit Nat’s gallery.