Is Your Bacterial Culture Still Growing? A Primer on OD­600 Measurements

May 29, 2018 Adrienne Huntress

This article is for anyone who has ever wondered, “How do I know when my bacterial culture is ‘done’ growing? When should I harvest my cells?” Bacterial Growth Curves Review Let’s start by reviewing the 4 basic phases of a bacterial growth curve: log phase, lag phase, stationary phase, and death phase (Figure 1). A…

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