zeiss_microscopy ZEISS Microscopy zeiss_microscopy July 13, 2018
zeiss_microscopy

We stained fixed #brain sections from somatosensory cortex of GAD67-GFP transgenic #mice with antibodies to somatostatin and parvalbumin. We observed that GFP expressing interneurons were colocalized with somatostatin but not with parvalbumin immuno- reactivity. This technique allowed us to classify the somatostatin containing interneurons into subtypes, based on their electro- physiological, morphological and neurochemical properties. #Cells pseudocolored red contain parvalbumin (PV). Cells pseudo- colored green express GFP. We can clearly identify that GFP #neurons are labeled with different markers through the multi-fluorescence channels of the LSM 510 #confocal #microscope. Dr. Hang Hu. Department of #Neurobiology & #Anatomy, West Virginia #University. Sensory Neuroscience Research Center. #zeiss #carlzeiss #microscopy

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