zeiss_microscopy ZEISS Microscopy zeiss_microscopy August 19, 2019

#ZEISS Celldiscoverer 7 with LSM 900 Airyscan 2. Lateral line primordium #migration and deposition of immature neuromasts in a #Zebrafish #embryo (Danio rerio). #Animals were anesthetized and embedded using low concentrated agarose in a glass bottom petridish. Initial camera based #imaging allowed for a quick and easy sample navigation (top) combining Phase Gradient #Contrast with fluorescence acquisition. Subsequent high #resolution imaging with Airyscan 2 in Multiplex mode was done on individual positions identified in the widefield image (white boxes). A) Maximum intensity projections of an immature neuromast (127 z planes). B) Maximum intensity projections of the lateral line primordium tip migrating through the #animal (155 z-planes). Green: LYN-eGFP (mebranes); Red: tagRFP-T-UTRCH (actin). The gentle and fast image acquisition that is inherent to the Airyscan 2 Multiplex mode is very benificial for this kind of application. The animal is unperturbed by the imaging while images with a very high signal to noise ratio as well as level of detail can be acquired at the same time. #microscopy #microscope #science #lifescience #research #superresolution #confocal #carlzeiss #biology

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