Assembly of a 3D dataset with ZEISS ZEN Correlative Array Tomography http://www.zeiss.com/zen-cat The dataset shows a Z-stack of macrophages expressing the protein huntingtin. Macrophages are used as a model system for the investigation of the Huntington’s disease. An overexpression of huntingtin results in an aggregation of the protein clearly visible within the Z-stack. An antibody was used against GFP-Huntingtin to locate plaques of huntingtin within the cells (Alexa Fluor 647, red). The nucleus is shown in blue (Hoechst). Courtesy of J. Caplan, E. Kmiec and S. Modla, University of Delaware, USA.
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