Lightsheet Z.1: Drosophila embryo, stage 10-14

April 8, 2014
Three Drosophila embryos were imaged with the detection optics 20x/1.0, each from 4 viewing angles every 6 minutes for 15 hours. The Multiview datasets were registered and fused into one 3D image using fiducials as landmarks; the channel with the fiducial signal was removed after registration. All embryos were embedded in 1 % low melting agarose and temperature was set to 25°C during the experiment. Image acquisition started before eYFP expression was visible and two of the Drosophila embryos showed expression of eYFP under control of the Dfd (deformed) enhancer during gastrulation (stage 10-14 and stage 10-17). eYFP is visible from stage 11 onwards in the mandibular and maxillary segment of the head in the posterior spiracles and denticle belts from stage 13. Autofluorescence in the yolk allows following gastrulation; the yolk ends up in the gut lumen. The gut of the embryo on the right starts to form constrictions at the end of the movie. At this point (stage 16), embryogenesis is almost finished and the embryo starts to move. Samples were provided by Dr. Julia Sellin, LIMES Institute, Bonn, Germany. http://www.zeiss.com/lightsheet
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Lightsheet Z.1: Drosophila embryo, stage 10-17
Lightsheet Z.1: Drosophila embryo, stage 10-17

Three Drosophila embryos were imaged with the detection optics 20x/1.0, each from 4 viewing angles every 6 ...