ZEISS Lightsheet Z.1: Spheroid imaging (landmark registration using fluorescent beads)

July 9, 2013
HBE human derived cell lines cultured as micro-tissues (DAPI staining in blue; fixed with PFA). Infection with human Adenovirus type 2 expressing GFP. Sample provided by the Greber Group, Vardan Andriasyan, Artur Yakimovich (University of Zürich). Imaged in the ZEISS Microscopy Labs, Munich. Dual side illumination, Views: 4 (90° rotation each), Landmark registration using fluorescent beads (small green dots surrounding the spheroid), Maximum Intensity Projection with Lightsheet Z.1 http://www.zeiss.com/lightsheet
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ZEISS Lightsheet Z.1: Spheroid imaging (intensity-based registration)
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HBE human derived cell lines cultured as micro-tissues (DAPI staining in blue; fixed with PFA). Infection w...