ZEISS Lightsheet Z.1: Thy1-EGFP M-line mouse hippocampus, optically cleared in LUMOS clearing agent

December 12, 2014
Thy1-EGFP M-line mouse hippocampus, optically cleared in LUMOS clearing agent. Imaged on ZEISS Lightsheet Z.1 modified for imaging of optically cleared specimens. http://www.zeiss.com/lightsheet The data set was acquired from a hemisphere whole mount. Imaging Parameters: ZEISS 20x/1.0 immersion lens for RI=1.45 (WD= 5.6 mm), Voxel Size: (x:y:z) 285 x 285 237 nm Volume Size: 4.12 x 0.466 x 2.11 mm (14755 x 1636 pixel and 8882 z-sections). Data processing and 3D rendering in arivis vision 4D software. Sample courtesy of O.Efimova, National Research Center, Kurchatov Institute, Moscow, Russia.
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ZEISS Lightsheet Z.1: Thy1-EGFP M-line mouse whole mount, optically cleared in LUMOS clearing agent
ZEISS Lightsheet Z.1: Thy1-EGFP M-line mouse whole mount, optically cleared in LUMOS clearing agent

Thy1-EGFP M-line mouse whole mount, optically cleared in LUMOS clearing agent, co-stained with Propidium Io...

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ZEISS Lightsheet Z.1: Thy1-EGFP M-line mouse whole mount, optically cleared in LUMOS clearing agent
ZEISS Lightsheet Z.1: Thy1-EGFP M-line mouse whole mount, optically cleared in LUMOS clearing agent

Thy1-EGFP M-line mouse whole mount, optically cleared in LUMOS clearing agent, co-stained with Propidium Io...