ZEISS Lightsheet Z.1: Thy1-EGFP M-line mouse whole mount, optically cleared in LUMOS clearing agent
Thy1-EGFP M-line mouse whole mount, optically cleared in LUMOS clearing agent, co-stained with Propidium Iodide. Imaged on ZEISS Lightsheet Z.1 modified for imaging of optically cleared specimens: http://www.zeiss.com/lightsheet Imaging Parameters: ZEISS 5x/0.16 lens in RI=1.45 (WD= 5.6 mm), Voxel Size: (x:y:z) approx. 1.3 µm x 1.3 µm x 1.75 nm Volume Size: 11.1 x 11 x 4.5 mm (8600 x 8500 pixel and 2588 z-sections). Data processing and 3D rendering in arivis vision 4D software. Sample courtesy of O.Efimova, National Research Center, Kurchatov Institute, Moscow, Russia.