ZEISS Lightsheet Z.1: Tribolium embryo development

August 14, 2013
This maximum intensity projection movie is of a Flour Beetle embryo (Tribolium castaneum). The egg is approximately 600 µm in length. This is a lateral view with anterior to the left and ventral up. Images were taken at 5 minute intervals and the total developmental time shown in the movie is 6 hrs and 30 minutes at a temperature of 29°C. The animal contains a GFP transgene which provides ubiquitous GFP expression in all nuclei (Tribolium strain courtesy of Sue Brown and Michalis Averof). At the start of the movie, the germband is just beginning to extend into the yolk, and the extra-embryonic membranes are closing over the ventral side. As the movie progresses, the embryo grows considerably, segments become morphologically visible, and the germband embryo extends to wrap around both the anterior and posterior end of the egg. Movie acquired during the 2013 MBL course on Embryology, Woods Hole. Kindly provided by Nipam H. Patel, Dept. of Molecular & Cell Biology, University of California, Berkeley. For more information visit http://www.zeiss.com/lightsheet
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