ZEISS LSM 710 & 780 NLO - Brain tissue

Principal (projection) neurons in cortex of a transgenic mouse that expresses YFP under the thy1 promoter (adult, YFP-H line). The fixed forebrain was removed and embedded in 8 % agarose with the caudal portion (cut surface) facing up. A region of the cortex was imaged from the cut surface to a depth of 260 microns using multiphoton excitation (930 nm). For more information visit http://www.zeiss.com/lsm710-nlo